Measurement of conjugated and unconjugated serum bile acid concentrations using 3 alpha-hydroxysteroid dehydrogenase.

Abstract

radioimmunoassay.9 However, when we applied the hydroxysteroid dehydrogenase-fluorimetric assay for bile acids'0 we obtained absurdly high recoveries from serum samples. This interference appeared to be associated with the use of Amberlyst A-15. When SP-Sephadex was substituted for Amberlyst A-IS," no interference with subsequent enzyme analysis was observed.