Proceedings of the National Academy of Sciences · 2007 · 192 citations · 19 references
Life SciencesDevelopmental BiologyGeneticsFish GeneticsReproductive BiologyReproductive GeneticsFish Developmental BiologyFish Reproductive BiologyGonadal Sex DifferentiationSex DeterminationSex DifferentiationSexual DifferentiationMale DifferentiationMale Sexual DevelopmentMolecular Sex DifferentiationSex Determination GenesSex Determination In FishSex Differentiation In VertebratesSex Differentiation PathwaysY Chromosome Function
Although the sex-determining gene SRY/Sry has been identified in mammals, homologues and genes that have a similar function have yet to be identified in nonmammalian vertebrates. Recently, DMY (the DM-domain gene on the Y chromosome) was cloned from the sex-determining region on the Y chromosome of the teleost fish medaka (Oryzias latipes). DMY has been shown to be required for the normal development of male individuals. In this study, we show that a 117-kb genomic DNA fragment that carries DMY is able to induce testis differentiation and subsequent male development in XX (genetically female) medaka. In addition, overexpression of DMY cDNA under the control of the CMV promoter also caused XX sex reversal. These results demonstrate that DMY is sufficient for male development in medaka and suggest that the functional difference between the X and Y chromosomes in medaka is a single gene. Our data indicate that DMY is an additional sex-determining gene in vertebrates.
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Male development of chromosomally female mice transgenic for Sry
Peter Koopman, John Gubbay, Nigel Vivian et al. · Nature · 1991 · 2.2K citations
DMY is a Y-specific DM-domain gene required for male development in the medaka fish
Masaru Matsuda, Yoshitaka Nagahama, Ai Shinomiya et al. · Nature · 2002 · 1.5K citations