FEMS Microbiology Letters · 1984 · 2.4K citations · 17 references
PharmacyBiochemistryMolecular BiologyChromatographic AnalysisNucleic Acid BiochemistryNucleic Acid ChemistryAnalytical ChromatographyChromatographyDna ChemistryLiquid ChromatographyNucleic Acid StructureNucleobase ChemistryDna Sequence CompositionHigh-performance Liquid ChromatographyHigh-performance Liquid Chromatography Method DevelopmentNucleotide ContentReverse-phase ChromatographyReverse-phase Liquid ChromatographyReversed-phase ChromatographyReversed-phase Liquid Chromatography
DNA base composition was determined by reversed-phase high-performance liquid chromatography (HPLC). DNA was hydrolysed into nucleosides with nuclease P1 and bacterial alkaline phosphatase. The mixture of nucleosides was applied to HPLC without any further purification. One determination by chromatography needed 2 µg of hydrolysed nucleosides and took only 8 min. The relative standard error of nucleoside analysis was less than 1%. The system described here gives a direct and precise method for determining DNA base composition.
17