Bismarck Brown As A Stain For Mucoproteins

Abstract

Bismarck brown has been used for many years as a stain for mucin. But some types of mucoprotein are so water-labile that they cannot be demonstrated by the aqueous or weak alcoholic solutions usually employed. It has been found that Bismarck brown in slightly acidified, strong alcoholic, solution stains mucin. A simple method is given for using this solution for staining water-stable mucoproteins. Another method is included in which full precautions are given for avoidance of water at all stages subsequent to fixation. This method must be used for the more water-labile mucoproteins. By the use of these methods, it has been possible to demonstrate a wide range of mucoproteins including those of the mast cells of Hardie and that of the zona pellucida of the graafian follicle.