Cytogenetics and Cell Genetics · 2008 · 185 citations · 0 references
Life SciencesGeneticsHematologyCytogeneticsHematologic OncologyHematopathologyCancer CytogeneticsChromosome AberrationsClinical CytogeneticsIn Situ HybridizationMinimal Residual DiseaseMolecular CytogeneticsChromosomal Aberration DetectionChromosome Aberration AnalysisDna In Situ HybridizationInterphase CytogeneticsLeukemia CytogeneticsMetaphase CytogeneticsMinimal Residual Disease MarkersMolecular Cytogenetic Diagnosis
Double fluorescence in situ hybridization was used to detect Philadelphia (Ph) chromosomes in interphase nuclei and metaphases of patients with chronic myeloid leukemia. Application of cosmid probes for 3' ABL and 5' BCR sequences gave better results than libraries for chromosomes 9 and 22. The present approach may provide an alternative method for monitoring minimal residual disease in Ph+ CML patients.