A simple automated solid-phase extraction procedure for measurement of 25-hydroxyvitamin D₃ and D₂ by liquid chromatography-tandem mass spectrometry

Abstract

Our simplified sample preparation and automated SPE method is suitable for the measurement of 25OHD(3) and D(2) in a routine laboratory environment. The system can process up to 300 samples per day with no cumbersome solvent evaporation step and minimal operator intervention.