Packed capillary supercritical fluid chromatography with mixed mobile phases and light‐scattering detection

Abstract

Abstract The combination of packed fused silica columns and the laser light‐scattering detector has been examined. A column, 10 cm × 0.32 mm i.d., packed with a 4‐μm C 18 material, was mounted in a drift tube that functioned as a column oven. The restrictor was positioned in the detector cell. Standard curves and slopes were obtained for 5–60 ng of trimyristin and Irgafos‐168 with 2.9 and 5.6 mol % of n‐propanol in carbon dioxide. With pressure programming at a constant modifier concentration, the relative standard deviations in peak areas were determined to be less than 4%, except for the 5‐ng injections. The reproducibility of retention times was better than 0.5%. Two applications are demonstrated.