Determination of relative abundance of splicing variants of <i>Oreochromis</i> glutamate receptors by quantitative reverse‐transcriptase PCR

Abstract

In this study, the relative abundance of splicing variants of Oreochromis non‐NMDA subtype glutamate receptors was studied by quantitative reverse‐transcriptase PCR (RT‐PCR). The relative expression level between the flip and flop transcripts of fGluR2α determined by quantitative RT‐PCR is apparently much higher than that estimated by sequence analysis of the cloned RT‐PCR products. Control studies were performed to demonstrate the accuracy of the application of quantitative RT‐PCR analysis in studying the relative abundance between the flip and flop transcripts of glutamate receptors.