Immunoassayable Insulin Content of Subcellular Fractions of Rat Islets¹

Abstract

Islets of Langerhans were isolated from rat islets by a modification of the collagenase digestion procedure. The islets were fractionated by centrifugation techniques and the presence of an enriched insulin secretion granule fraction was shown as well as the quantitative distribution of insulin in other fractions. The content of insulin relative to other proteins in individual fractions was estimated. Current literature on insulin content of islets and pancreas was discussed with respect to these results. (Endocrinology85: 218, 1969)