Rapid Communications in Mass Spectrometry · 1996 · 71 citations · 0 references
PharmacyBiochemistryPharmacologyAnalytical ToxicologyBiochemical PharmacologyMass Spectrometric AnalysisBioanalytical Method ValidationGenotoxicity AssessmentOxidative Damage To BiomoleculesToxicological Drug ScreeningDerivatizationElectrospray IonizationIntraperitoneal AdministrationLiquid Chromatography-mass SpectrometryLiquid Chromatography-mass Spectrometry AnalysisOxidative Damage BiomarkersOxidative Dna DamageTandem Mass Spectrometry
A method based on liquid chromatography (LC) combined with electrospray ionization tandem mass spectrometry for the analysis of the frequency of formation of 8-hydroxydeoxyguanosine (80HdGuo), a biomarker of oxidative damage to DNA, has been developed. This analytical technique has the advantage over gas chromatography combined with mass spectrometry of not requiring analyte derivatization, and over LC combined with ultraviolet and electrochemical detection of being chemospecific. The method was evaluated by determining increased frequency of formation of 80HdGuo in male Sprague-Dawley rats given a single intraperitoneal dose of Adriamycin compared to control rats. Detection of one oxidized deoxyguanosine per 5 x 10(5) deoxyguanosines was readily achieved.