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PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloning

Cortland Lohff, Kemp B. Cease

Nucleic Acids Research · 1992 · 31 citations · 8 references

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Abstract

Cortland J. Lohff, Kemp B. Cease; PCR using a thermostable polymerase with 3' to 5' exonuclease activity generates blunt products suitable for direct cloni

References

8

Molecular cloning: A laboratory manual

Prescott L. Deininger · Analytical Biochemistry · 1990 · 86.2K citations

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Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products

Douglas A. Marchuk, Mitchell L. Drumm, Ann M. Saulino et al. · Nucleic Acids Research · 1991 · 1.2K citations · Full text

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Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases

James M. Clark · Nucleic Acids Research · 1988 · 844 citations · Full text

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A simple method for site-directed mutagenesis using the polymerase chain reaction

Anne Hemsley, Norman Arnheim, Michael D. Toney et al. · Nucleic Acids Research · 1989 · 622 citations · Full text

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A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors

Timothy A. Holton, Michael W. Graham · Nucleic Acids Research · 1991 · 385 citations · Full text

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