British Standard Poliomyelitis Antisera Types 1, 2, and 3

Abstract

MErBxsf 1549 polioviruses; and (b) to avoid the potential neutralizing effect of monkey antisera against simian viruses. Undiluted culture fluid was mixed with an equal quantity of the typespecific rabbit antiserum, and after an incubation period of one hour at room temperature 0.2 ml. was distributed in each of 10 monkey-kidney-culture tubes. At the end of seven days the medium is changed to permit observation of the tubes for another seven days for the detection of minimal amounts of non-poliovirus cytopathogenic agents.