Method to isolate polyribosomal mRNA from scarce samples such as mammalian oocytes and early embryos

Abstract

We report here the successful use of a method to profile mRNA present in the polyribosomal fraction obtained from as little as 75 mammalian oocytes. Polyribosomal mRNA fractionation thus provides a new tool for studying gametogenesis and early development with better representation of the underlying physiological status.