Physical crosslinking of collagen fibers: Comparison of ultraviolet irradiation and dehydrothermal treatment

TLDR

We have content for each. Background: two sentences: first line: "Abstract The strength, resorption rate, and biocompatibility of collagenous biomaterials are profoundly influenced by the method and extent of crosslinking." second: "Partial loss of the native collagen structure may influence attachment, migration, and proliferation of cells on collagen fiberbased ligament analogs." Combine into one sentence summarizing field context: "The strength, resorption rate, and biocompatibility of collagenous biomaterials are profoundly influenced by the method and extent of crosslinking, and loss of native collagen structure can affect cell attachment, migration, and proliferation on collagen fiber–based ligament analogs." That is one sentence. Purpose: we have two Purpose lines: first: "We compared the effects of two physical crosslinking methods, ultraviolet irradiation (UV) (254 nm) and dehydrothermal (DHT) treatment, on the mechanical properties and molecular integrity of collagen fibers extruded from an acidic dispersion of type I bovine dermal collagen." second: "These issues are currently being addressed in our laboratory." The second is vague; but we can incorporate main aim: "The study compares ultraviolet irradiation and dehydrothermal treatment on the mechanical properties and molecular integrity of collagen fibers." That covers purpose. One sentence. Mechanism: The same line as Purpose/Mechanism: "We compared the effects of two physical crosslinking methods, ultraviolet irradiation (UV) (254 nm) and dehydrothermal (DHT) treatment, on the mechanical properties and molecular integrity of collagen fibers extruded from an acidic dispersion of type I bovine dermal collagen." That is mechanism: describing methods.

Abstract

Abstract The strength, resorption rate, and biocompatibility of collagenous biomaterials are profoundly influenced by the method and extent of crosslinking. We compared the effects of two physical crosslinking methods, ultraviolet irradiation (UV) (254 nm) and dehydrothermal (DHT) treatment, on the mechanical properties and molecular integrity of collagen fibers extruded from an acidic dispersion of type I bovine dermal collagen. Collagen fibers exposed to UV irradiation for 15 min had ultimate tensile strength (54 MPa) and modulus (184 MPa) values greater than or equivalent to values for fibers crosslinked with DHT treatment for 3 or 5 days. UV irradiation is a rapid and easily controlled means of increasing the mechanical strength of collagen fibers. Characterization of collagen extracted from the crosslinked samples by dilute acetic acid and limited pepsin digestion indicate that both UV and DHT treatments cause fragmentation of at least a portion of the collagen molecules. Partial loss of the native collagen structure may influence attachment, migration, and proliferation of cells on collagen fiberbased ligament analogs. These issues are currently being addressed in our laboratory. © 1995 John Wiley & Sons, Inc.