Ribonucleic Acid Content of Encephalomyocarditis Virus

Abstract

Summary The RNA content of encephalomyocarditis virus was found to be 31·7 %, a figure obtained by combining results from nitrogen and phosphorus determinations which gave 32·0 %, from extinction measurements at 260 nm. on perchloric acid hydrolysates which gave 31·3% and from orcinol determinations which gave 31·8%. Specific extinction coefficients obtained were E1 em 1%. at 260 nm. = 77·4 for the virus in 0·1 m-KCI+ 0·02 m-phosphate buffer, pH 8·0, and E1 cm 1% at 260 nm.= 221 for its RNA in 0·0 m-tris + HCl, pH 7·2.