Redox Regulation in <i>Bacillus subtilis</i> : The Bacilliredoxins BrxA(YphP) and BrxB(YqiW) Function in De-Bacillithiolation of <i>S</i> -Bacillithiolated OhrR and MetE

Abstract

We demonstrate that BrxA and BrxB function in the reduction of BSH mixed protein disulfides with two natural substrates (MetE, OhrR). These results provide biochemical evidence for a new class of bacterial redox-regulatory proteins, the bacilliredoxins, which function analogously to glutaredoxins. Bacilliredoxins function in concert with other thiol-disulfide oxidoreductases to maintain redox homeostasis in response to disulfide stress conditions.