Visualizing Metabolically Labeled Glycoconjugates of Living Cells by Copper‐Free and Fast Huisgen Cycloadditions

Abstract

CuI-free click: 4-Dibenzocyclooctynol reacts, in the absence of a CuI catalyst, exceptionally fast with azido-containing saccharides and amino acids to give stable triazoles. A biotin-modified derivative is ideally suited for visualizing and tracking glycoconjugates of living cells that are metabolically labeled with azido-containing monosaccharides (see image). Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z705456_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.