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IFN‐γ Restores HIV‐ and Non‐HIV‐Specific Cell Mediated Immune Response <i>In Vitro</i> and Its Activity is Neutralized by Antibodies from Patients with AIDS

DOI

13

Citations

0

References

1996

Year

Maria Antonia De Francesco, Arnaldo Caruso, Francesco Dima, A. CANTALAMESSA, Angelo Donato Canaris, Susanna Folghera, Simona Fiorentini, Gigliola Flamminio, Stefano Licenziati, L. Peroni,
Scandinavian Journal of Immunology

Abstract

The addition of IFN-gamma to cultures of peripheral blood mononuclear cells (PBMCs) obtained from asymptomatic HIV-infected patients increased cell proliferation in response to HIV envelope synthetic peptides (Env), influenza A virus (VIRUS), and allogeneic lymphocytes (ALLO) but not to phytohaemagglutinin (PHA) stimulation. F(Ab)2 fragments of IgG purified from the sera of HIV-seropositive patients specifically interfered with IFN-gamma-induced cell proliferation in response to recall antigens. Neutralization of the lymphokine activity was found to be sustained by specific IFN-gamma antibodies. Data obtained demonstrate that IFN-gamma can restore the cell-mediated immunity of a number of asymptomatic HIV+ individuals in vitro, while IFN-gamma antibodies present in sera of patients with AIDS interfere with the activity of the lymphokine.