Genetic Manipulation of Rubisco: <i>Chromatium vinosum rbc</i>L is expressed in <i>Nicotiana tabacum</i> but does not form a functional protein

Abstract

Summary N. tabacum lines that lacked functional Rubisco were transformed with plasmids encoding a chloroplast transit peptide in frame with C. vinosum rbc L and stable transformants generated. However, the transgene was transcribed at a low level and no Rubisco activity or C. vinosum large subunits were detectable in any line.