Transmission features <i>of Mai de Río Cuarto virus</i> in wheat by its planthopper vector <i>Delphacodes kuscheli</i>

Abstract

Summary Mal de Rio Cuarto virus (MRCV, Fijivirus ) infects maize ( Zea mays ) and other gramineae, producing significant losses in Argentina. Although MRCV mainly affects maize, other cereals, such as wheat, serve as potential virus reservoirs and hosts for the planthopper vector Delphacodes kuscheli. Aspects of the virus‐vector relationships were elucidated by studying the minimum periods of acquisition access (AAP min ), latency (LP min ) and inoculation access (IAP min ) in wheat ( Triticum aestivum L.). Trials were conducted under controlled conditions of temperature (24 ± 1°C), photoperiod (12 h light) and humidity (50 ± 5%). The results show that the AAPmin was 5 h, LPmin was 10 days (even though, the median latent period was between 16 and 17 days) and IAPmin was 30 min. Our experiments have demonstrated, for the first time, a persistent manner of virus transmission. No differences were detected in transmission ability between males and females. The implication of these results on virus taxonomy and epidemiology are discussed.