Platelet-rich fibrin modulates cell proliferation of human periodontally related cells in vitro

TLDR

Platelet‑rich fibrin (PRF) is a second‑generation platelet concentrate that yields fibrin membranes rich in platelets and growth factors, yet its biological effects on periodontally related cells remain poorly characterized. The study aimed to provide clear evidence for clinical use of PRF by examining its effects on human gingival fibroblasts, periodontal ligament cells, oral epithelial cells, and osteoblasts. PRF was prepared from blood of 10 healthy volunteers by centrifugation at 3000 rpm for 12 min, and the resulting membranes were tested on primary GFs and PDL cells, the GNM epithelial line, and the U2OS osteoblast line using trypan blue and tetrazolium bromide reduction assays to assess viability and proliferation. PRF did not alter cell viability but stimulated proliferation of osteoblasts (135 % of control), PDL cells (130 %), and GFs (120 %) over a 3‑day culture period, indicating cell‑type‑specific actions that may benefit periodontal regeneration.

Abstract

Background/Purpose Platelet-rich fibrin (PRF) is a second-generation platelet concentrate which allows one to obtain fibrin membranes enriched with platelets and growth factors, after an anticoagulant-free blood harvest. However, limited information is currently available concerning the biologic effects of PRF on periodontally related cells. To provide clear evidence for the clinical use of PRF, we investigated the biologic effects of PRF on human gingival fibroblasts (GFs), periodontal ligament (PDL) cells, oral epithelial cells, and osteoblasts. Materials and methods Blood collection was carried out on 10 healthy volunteers. PRF was obtained by centrifugation at 3000 rpm for 12 minutes with a PC-02 table centrifuge. Primary cultured human GFs and PDL cells, the GNM oral epithelial cell line, and the U2OS osteoblast cell line were used to evaluate cell viability and proliferation resulting from PRF according to trypan blue and tetrazolium bromide reduction assays. Results PRF did not interfere with cell viability of periodontally related cells (P > 0.05). PRF stimulated cell proliferation of osteoblasts (135% of the control), PDL cells (130% of the control), and GFs (120% of the control) during a 3-day culture period (all P Conclusion Our results suggest that PRF modulates cell proliferation in a cell type- specific manner. These cell type-specific actions may be beneficial for periodontal regeneration.