Cell Proliferation · 1969 · 181 citations · 19 references
Life SciencesCell BiologyHematologyCell ProliferationRadiobiologyStem Cell BiologyBone Marrow PhysiologyHematopoietic Stem Cell BiologyHematopoietic Stem CellsRadiation Effects On Stem CellsStem Cell PhysiologyStem Cell ProliferationHematopoietic Stem Cell MaintenanceHematopoietic Stem Cell Radiation ResponseOvershootPopulation OvershootRadiation Effects On The Hematopoietic SystemStem Cell Proliferation After IrradiationStem Cell Proliferation AssaysStem Cell Self-renewal After Irradiation
ABSTRACT A comparison of the exocolonizing and autorepopulating tests for haemopoietic stem‐cell assay indicate that the ‘overshoot’in splenic colony formation, observed 12–14 days after 150 rad total‐body radiation (TBR), only occurs with the auto‐repopulation assay. The explanation is that the priming dose of 150 rad increases the absolute seeding rate of stem cells from the marrow. A seeding rate significantly greater than normal can ‘take’only if the spleen is available—it can expand and accommodate stem cells while the bone marrow cannot. If, however, the absolute number of colony‐forming cells are decreased in the femur, a relative increase in seeding rate can take place even in the splenectomized animal. Evidence is presented concerning the different turnover states of exo‐ and autorepopulating stem cells (CFU) and those responsible for erythropoietic response (ERC), and the precursors of agar colony‐formers.
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THE GROWTH OF MOUSE BONE MARROW CELLS <i>IN VITRO</i>
TR Bradley, D Metcalf · Immunology and Cell Biology · 1966 · 1.9K citations