A Practical Guide to Deconvolution of Fluorescence Microscope Imagery

Abstract

Over the past decade, deconvolution of 3D light optical microscopy data has advanced from an obscure technique employed by only a few dedicated souls to a routine method that is now available with all modern microscope systems. Dramatic increases in computer power, algorithm sophistication, and software ease of use have brought the power of deconvolution to the general microscope user, and processing large 3D datasets is no longer a rate-limiting step in the imaging process.