Publication | Closed Access
An improved method for the extraction of adenosine triphosphate from marine sediment and seawater
66
Citations
0
References
1978
Year
Improved MethodEngineeringMarine ChemistryBioenergeticsBioanalysisBioremediationMarine PollutionMicrobial EcologyAnalytical ChemistryEnvironmental MicrobiologyBiological OceanographyMolecular GeochemistryChromatographyChemical OceanographyBiochemistryAdenosine TriphosphateMarine SedimentMarine BiotechnologyEstuarine GeochemistryEnvironmental EngineeringBicarbonate BuffersMarine MaterialsStationary Phase BacteriaMicrobiologyMarine BiologyMedicineCoastal Geochemistry
A method is described for extracting adenosine triphosphate (ATP) from both marine sediment and seawater with MeIlvaine (phosphate‐citrate) buffer at pH 7.70. This method compares favorably with techniques using tris and bicarbonate buffers and avoids the disadvantages of mobilized cations produced by an acidic extracting medium. Recovery of ATP added as stationary phase bacteria was 94.5% from sediment and 97% from seawater.