Cytofluorometric measurements of nuclear DNA in adventitious buds and shoots of <i>Picea abies</i> regenerated in vitro

Abstract

Nuclear DNA content of adventitious buds and shoots of Picea abies (L.) Karst. developed in vitro was compared to that of buds collected from field‐grown trees. Protoplasts were isolated from the different tissues and after fixation and staining with ethidium bromide the DNA content of their interphase nuclei was determined cytofluorometrically. The DNA pattern of the different tissues was within the same range and had the same distribution with one main peak.