Fragment Sizes Produced from T5 Bacteriophage DNA Molecules by Acid Deoxyribonuclease

Abstract

The action of acid deoxyribonuclease on T5 bacteriophage DNA results in a "random distribution" of sizes of duplex fragments as judged by electron microscopy. No preferred subunit size resulting from the "single hit" action can be detected down to lengths of 0.1 micron or less. The results are in agreement with the coexistence of a "single-hit" and a "double-hit" kinetics.