EXPRESSION OF COMPLEMENT REGULATORY PROTEINS ON ISLETS OF LANGERHANS: A COMPARISON BETWEEN HUMAN ISLETS AND ISLETS ISOLATED FROM NORMAL AND hDAF TRANSGENIC PIGS1

Abstract

Porcine and human islet cells express species-restricted complement regulatory proteins, with the human islet cells expressing mainly hCD59. A low expression of hDAF was detected on islet cells from one of five hDAF TG pigs. These islet cells displayed reduced islet cell cytotoxicity in fresh human serum. We conclude that protection from complement-mediated lysis will be important in the context of intraportal pig-to-human islet transplantation, and expression of a human RCA on islet cells should be beneficial in this context.