(2‐Cyano‐1,1‐dimethylethoxy)bis(diethylamino)phosphine: A convenient reagent for the synthesis of DNA fragments

Abstract

Abstract The phosphitylating reagent (2‐cyano‐1,1‐dimethylethoxy)bis(diethylamino)phosphine proved to be very effective for the in situ preparation of d‐nucleoside phosphoramidites. These latter compounds could be used for the introduction of 3′,5′‐internucleotide bonds and for the synthesis of DNA fragments both in solution and on a solid support. Removal of the 2‐cyano‐1,1‐dimethylethyl protective group at P(V) could easily be effected by ammonolysis.