Fasga: A new polychromatic method for simultaneous and differential staining of plant tissues

Abstract

SUMMARY A new method based on the simultaneous action of Safranin and Alcian Blue (or green) is reported. The main advantages of this method are its simplicity and the absence of differentiation processes. Free‐hand, frozen and paraffin sections can be stained with this technique. Sections are placed in 10% sodium hypochlorite for 1–5 min, rinsed in 5% acetic acid and stained for 10 min. Sections are then washed in distilled water for 1 min, rinsed in distilled water for 5 min and mounted.