Human reserve pluripotent mesenchymal stem cells are present in the connective tissues of skeletal muscle and dermis derived from fetal, adult, and geriatric donors

TLDR

The study profiles 13 cell surface markers on human reserve stem cells from connective tissues of fetal, adult, and geriatric donors. Stem cells were isolated from dermis and skeletal muscle connective tissues of fetal, mature, and geriatric humans and characterized using an insulin/dexamethasone phenotypic bioassay. All populations contained lineage‑committed myogenic, adipogenic, chondrogenic, and osteogenic progenitors, as well as pluripotent stem cells capable of differentiating into muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells; adult cells expressed CD34 and CD90 but were negative for a broad panel of immune and hematopoietic markers. © 2001 Wiley‑Liss, Inc., Anat Rec 264:51–62.

Abstract

Abstract This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric humans. An insulin/dexamethasone phenotypic bioassay was used to determine the identity of the stem cells from each population. All populations contained lineage‐committed myogenic, adipogenic, chondrogenic, and osteogenic progenitor stem cells as well as lineage‐uncommitted pluripotent stem cells capable of forming muscle, adipocytes, cartilage, bone, fibroblasts, and endothelial cells. Flow cytometric analysis of adult stem cell populations revealed positive staining for CD34 and CD90 and negative staining for CD3, CD4, CD8, CD11c, CD33, CD36, CD38, CD45, CD117, Glycophorin‐A, and HLA DR‐II. Anat Rec 264:51–62, 2001. © 2001 Wiley‐Liss, Inc.