Comparison of plasma from healthy nonsmokers, smokers, and lung cancer patients: Pattern-based differentiation profiling of low molecular weight proteins and peptides by magnetic bead technology with MALDI-TOF MS

Abstract

CONTEXT: Smoking is the major contributor of lung cancer (LC), which accounts for millions of death. OBJECTIVE: This study focused on the correlation between the proteomic profiling of LC patients, and healthy nonsmokers and smokers. METHOD: Pattern-based peptide profiling of 186 plasma samples was performed through reversed-phase chromatography-18 magnetic bead fractionation coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis and resulted data were evaluated statistically by ClinProTool. RESULTS: Marker peaks at m/z 1760, 5773, 5851, 2940, and 7172 were found with an excellent statistical figure. CONCLUSION: Selected marker peaks can be served as a differentiated tool of LC patients with high sensitivity and specificity.