FEBS Letters · 1992 · 17 citations · 12 references
Life SciencesBiochemistryMicrobiologyCrystal StructureEnzymologyStructural BiologyCrystal ChemistryCrystal SystemsCrystallization AnalysisCrystallization ProcessesCrystallographyMacromolecular AssemblyAminoacyl-transfer Ribonucleic Acid Synthetase ComplexesAminoacyl-transfer Ribonucleic Acid Synthetase StructureAminoacyl-trna SynthetasesCrystal FormCrystallographic CharacterizationUnit Cell DeterminationUnit Cell GeometryUnit Cell Parameters
Two distinct complexes between seryl-tRNA synthetase and tRNA(Ser) from Thermus thermophilus have been crystallized using ammonium sulphate as a precipitant. Form III crystals grow from solutions containing a 1:2.5 stoichiometry of synthetase dimer to tRNA. They are of monoclinic space group C2 with unit cell dimensions a = 211.6 A, b = 126.8 A, c = 197.1 A, beta = 132.4 degrees and diffract to about 3.5 A. Preliminary crystallographic results show that the crystallographic asymmetric unit contains two synthetase dimers. Form IV crystals grow from solutions containing a 1:1.5 stoichiometry of synthetase dimer to tRNA. They are of orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions a = 124.5 A, b = 128.9 A, c = 121.2 A and diffract to 2.8 A resolution. Preliminary crystallographic results show that these crystals contain only one tRNA molecule bound to a synthetase dimer.
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