Ionic Liquid-Coated Enzyme for Biocatalysis in Organic Solvent

Abstract

Ionic liquid-coated enzyme (ILCE) is described as a useful catalyst for biocatalysis in organic solvent. An ionic liquid, [PPMIM]-[PF6] (1, [PPMIM] = 1-(3‘-phenylpropyl)-3-methylimidazolium), which is solid at room temperature and becomes liquid above 53 °C, was synthesized in two steps from N-methylimidazole. The coating of enzyme was done by simply mixing commercially available enzyme with 1 in the liquid phase above 53 °C and then allowing the mixture to cool. A representative ILCE, prepared with a lipase from Pseudomonas cepacia, showed markedly enhanced enantioselectivity without losing any significant activity.