Single‐Molecule DNA Biosensors for Protein and Ligand Detection

Abstract

Transcription factors (TFs) control gene expression and are promising biomarkers for diseases. In a new, robust, and sensitive single-molecule fluorescence assay TFs and associated small molecules (such as nucleotides and sugars) are detected based on DNA coincidence of the two halves of the binding site (see picture; S=stoichiometry). The assay is compatible with measurements in solution, on solid supports, and even in complex biological samples.