Spectrally resolved absolute fluorescence cross sections for bacillus spores

Abstract

Absolute fluorescence cross sections for Bacillus subtilis and B. cereus bacterial spores as both aqueous suspensions and aerosols were measured at a number of excitation wavelengths between 228 and 303 nm. The fluorescence was spectrally resolved at each excitation wavelength. We found that the optimum excitation wavelength for spore fluorescence is between 270 and 280 nm. The fluorescence cross section for aqueous suspensions is four times larger than for dry aerosols when measured under similar conditions. Measurements on wet aerosols showed an increase in fluorescence cross section over dry aerosols, indicating an enhancement of the fluorescence when the bacterial spores are wet. Mie scattering cross sections at 90 degrees to the direction of the incident radiation and extinction cross sections as a function of wavelength for B. subtilis suspensions and fluorescence cross sections for tryptophan are also reported.