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EFFECT OF 3′:5′-CYCLIC AMP ON NUCLEAR AND MITOCHONDRIAL RNA SYNTHESIS OF RAT ADRENOCORTICAL CELLS
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1972
Year
Cellular PhysiologyAdrenal GlandPituitary GlandNeuroendocrine MechanismMetabolismSteroid MetabolismMolecular PhysiologyBiochemistryEndocrine MechanismNervous SystemEndocrinologyPharmacologyCell BiologyMitochondrial FunctionPhysiologyHigh Resolution AutoradiographyTracer IncorporationMedicine3′:5′-Cyclic Amp
ABSTRACT The effect of 3′:5′-cyclic AMP (cAMP) on the incorporation of 3 H-uridine into adrenocortical cells of hypophysectomized rats was investigated by high resolution autoradiography. The quantitative analysis of autoradiographs shows that cAMP, like ACTH, enhances the tracer incorporation into both nuclei and mitochondria. These findings are discussed in relation to the results of various investigations, indicating that cAMP functions as an intracellular mediator of the long-term trophic action of ACTH on the adrenal cortex. It is suggested that the mechanism of this action of cAMP consists in the stimulation of both nuclear and mitochondrial RNA synthesis. Furthermore, the possibility that the trophic action of ACTH on the adrenal gland involves other intracellular mediators, as well as cAMP, is suggested.