Riboswitches for Enhancing Target Gene Expression in Eukaryotes

Abstract

Regulating gene expression: The thiamine pyrophosphate-dependent thiA riboswitch naturally downregulates gene expression upon thiamine pyrophosphate binding in eukaryotes. In the current study, we used this as the basis to engineer an “on riboswitch” that can upregulate gene expression. The riboswitches developed here may be useful as tools for gene regulation.