<i>In‐vitro</i> Crystallization of Calcium Carbonate in the Presence of Biological Additives — Comparison of the Ammonium Carbonate Method with Double‐Diffusion Techniques

Abstract

Abstract The biomineralization process was simulated in vitro by the precipitation of calcium carbonate in the presence of two biological additives: bovine serum albumin (BSA) and soluble matrix from the shell of the freshwater snail Biomphalaria glabrata . The crystallization was carried out with the classical ammonium carbonate diffusion method and with a double diffusion setup under pH‐controlled conditions. The inhibiting effects of BSA and soluble shell matrix could only be observed in the double diffusion setup, but not with the ammonium carbonate method.