Modified phosphotriester method for chemical synthesis of ribooligonucleotides. Part I. Synthesis of riboundecaadenylate and two fragments constituting the sequence of R-17 translation control signal

Abstract

A modified phosphotriester method has been successfully applied for the chemical synthesis of ribooligonucleotides. The starting material is a fully protected ribomononucleoside containing a 3′-phosphotriester group 5. The coupling reaction is performed using mesitylenesulfonyl tetrazole and purification of the product achieved using reversed phase column chromatography. The effectiveness of this method has been demonstrated by achieving an efficient and rapid synthesis of r-A 7 , r-A 11 , r5′-AAACAUGAGGA-3′, and r5′-UUACCCAUGU-3′ (R-17, translation control sequence).