Peptide synthesis enzymatically catalyzed in a biphasic system: Water–<i>water–immiscible</i> organic solvent

Abstract

Abstract Synthesis of a peptide bond is suggested to be enzymatically catalysed in a biphasic system “water– water‐immiscible organic solvent”. The pH dependence of the apparent equilibrium constant is studied for synthesis of N ‐acetyl‐ L ‐tryptophanyl‐ L ‐leucine amide from N ‐Acet‐Trp and L ‐Leu‐NH 2 . The reaction was performed in the biphasic system ethyl acetate plus water [from 2 to 2% (v/v)] in the presence of α‐chymotrypsin. The suggested approach is preparative value: with the stoichiometric ratio of the reagents, [ N ‐Acet‐ L ‐Trp] = [ L ‐Leu‐NH 2 ] = 2 × 10 −3 M , the yield is practically 100% (in water, with other conditions being the same, the yield is not over 01.%).