Journal of Cellular Biochemistry · 2011 · 10 citations · 28 references
MedicineBiotechnologyMolecular BiologyBone MetabolismRegenerative MedicineStem Cell BiologyBone DevelopmentBone FormationBone RegenerationMesenchymal DifferentiationMesenchymal Stem Cell TherapyOsteogenesisBmp-induced Cell DifferentiationBone Injury RepairBone Marrow - Derived CellsBone Marrow Mesenchymal Stem CellsEscherichia ColiEscherichia Coli Expression SystemsOsteoinductive SignalingPhosphatase Enzyme Activity
Bone morphogenetic proteins (BMPs) induce differentiation of mesenchymal cells to cartilage and bone. We cloned BMP4 and BMP7 cDNAs from human placenta and fetal cartilage cells, respectively, and used an Escherichia coli expression system to produce recombinant BMP4 and BMP4/7 proteins. Differentiation of primary cultures of bone marrow stem cells (BMSC) treated with BMP4 or BMP4/7 was evaluated by Von Kossa staining and by determining alkaline phosphatase activity and osteocalcin level. BMP4/7-induced BMSC differentiation more potently than BMP4. We showed that BMP4/7 fusion protein expressed in E. coli is biologically active and is a novel strategy to treat bone injury in a clinical setting.
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