Optical sectioning structured illumination microscopy with enhanced sensitivity

Abstract

A new approach to reconstructing an optically sectioned image using structured illumination is presented. Compared to the algorithm proposed by Neil et al (1997 Opt. Lett. 22 1905), this method uses the same number of images to construct a final image with a flat linear transfer function at the cost of slightly more complexity in the reconstruction algorithm. Compared to other optical sectioning algorithms using structured illumination, this approach produces images with higher contrast and better image fidelity at low signal intensities.