Target‐Catalyzed Transfer Reactions for the Amplified Detection of RNA

Abstract

Strong signals: The RNA-catalyzed transfer of a biotin reporter was used to preamplify a signal that was detected in an enzyme-based readout, thereby increasing its sensitivity. The advantages of combining the covalent attachment of biotin and preamplification were demonstrated by the detection of 500 attomol HIV RNA. The flexibility requirements of DNA- and RNA-catalyzed transfer reactions were tested. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z801355_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.