Construction of an ASD+ Expression-Cloning Vector: Stable Maintenance and High Level Expression of Cloned Genes in a Salmonella Vaccine Strain

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

Ulrich K. Laemmli · Nature · 1970 · 251K citations

Protein Assembly, Natural Sciences, Structural Proteins +8

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Immunocytochemical Technique, Glycobiology, Polyacrylamide Gels +19

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.8K citations · Full text

Genetics, Bacteriology, Bacteriophage +18

The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers

Jeffrey Vieira, Joachim Messing · Gene · 1982 · 6.1K citations

M13mp7-derived System, Dna Sequencing, Engineering +15

Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid

Annie Chang, Stanley N. Cohen · Journal of Bacteriology · 1978 · 4.3K citations · Full text

Amplifiable Multicopy Dna, Engineering, Dna Analysis +20