PLANT PHYSIOLOGY · 1987 · 380 citations · 0 references
Life SciencesPlant BiologyPlant SciencesPhotosynthesisPlant BiochemistryPlant PhysiologyChloroplast BiochemistryCitric Acid CycleEnergy MetabolismLeaf PhysiologyPlant MetabolismPlant MetabolomicsChloroplast Enzyme RegulationChloroplast MetabolismChloroplast MetabolomicsPlant Metabolite ProfilingSubcellular Metabolite LocalizationSucrose BiosynthesisSucrose Gradient CentrifugationSucrose Metabolism Enzymes
The alterations of subcellular metabolite levels during the day in spinach leaves have been investigated using nonaqueous density gradient centrifugation to separate chloroplasts, cytosol, and vacuole. The results provide direct evidence for the role of sucrose phosphate synthase and cytosolic fructose 1,6-bisphosphatase in regulating sucrose synthesis in leaves and also show that the phosphate translocator is kinetically limiting in vivo.