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Role of DNA definite structural elements in interaction with repair enzyme uracil‐DNA glycosylase

DOIFull Paper Access

10

Citations

0

References

1998

Year

N. L. Vasilenko, Olga V. Vorobjeva, Evgeny M. Volkov, Tatjana S. Oretskaya, Galina A. Korshunova, Georgy A. Nevinsky
IUBMB Life

Abstract

Interaction of different oligodeoxyribonucleotides (oligos), their analogs and oligonucleopeptide with uracil-DNA glycosylase (UDG) from human placenta was investigated. It is shown that there is no considerable contribution of heterocyclic bases of DNA to UDG-substrate binding but the UDG interaction with some DNA phosphate groups is necessary for enzyme-substrate recognition. However the phosphate group adjacent to single dU from the 3'-end in oligo is not involved into the electrostatic contact with UDG. It is found that UDG has the high affinity to its reaction product. An oligonucleotide containing a single 2'-deoxy-2'-aminouridine is a non-hydrolyzable substrate analog for UDG.