A Duplex Quantitative Polymerase Chain Reaction Assay Based on Quantification of <i>α-Methylacyl-CoA Racemase</i> Transcripts and <i>Prostate Cancer Antigen 3</i> in Urine Sediments Improved Diagnostic Accuracy for Prostate Cancer

Abstract

Urinary AMACR and PCA3 tests were superior to a serum prostate specific antigen test for detecting prostate cancer. Their combined use in a dual marker test further improved sensitivity and accuracy, and could serve as a surveillance test after repeat negative prostate biopsies.