Synthesis of Magnetic Microspheres with Immobilized Metal Ions for Enrichment and Direct Determination of Phosphopeptides by Matrix‐Assisted Laser Desorption Ionization Mass Spectrometry

Abstract

Core/shell structured magnetic silica microspheres with immobilized Fe3+ (see image) are prepared with a simple method. Using a magnetic field, the microspheres are quickly, efficiently, and specifically enriched. Phosphopeptide identification can then be achieved through mass spectroscopy. This is a new method for the enrichment of phosphopeptides and opens up the possibility of other new applications using the microspheres. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2006/c1546_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.