The Pulsed Dye Laser as a Light Source for the Fluorescent Antibody Technique

Abstract

Recently, the substitution of conventional light sources for fluorescence microscopy by laser system have yielded promising results. The system reported here is capable of creating fluorescent images by excitation with defined pulses of coherent light. Photographic recordings of fluorescent objects have been produced with exposures of less than 10 μsec. No significant amount of fluorescence bleaching was noted, even after illumination for 50 μsec. The laser light source holds a promise for improvement in micro‐fluorometry, and is discussed with special reference to quantification in immunofluorescence.