Differentiation of some pathovars of <i>Pseudomonas syringae</i> with monoclonal antibodies¹

Abstract

Two stable hybridoma clones secreting antibodies specific for three pathovars of Pseudomonas syringae were obtained from a fusion of murine myeloma cells with spleen cells of BALB/c mouse immunized with P. syringae pv. savastanoi. Undiluted hybridoma culture medium reacted strongly in indirect ELISA tests with 20 strains of pv. savastanoi , 10 strains of pv. tomato , and 3 strains of pv. papulans. There were no reactions with 23 (of 24) strains of pv. glycinea , three strains each of pvs pisi and tabaci , two strains of pv. tagetis and one each of pvs lachrymans and aptata. Hybridomas also reacted positively with six of 16 strains of pv. syringae and with one of three strains of pv. phaseolicola.